动物造模,药效评价,非细菌性前列腺炎 z-bio 2024-11-18 363

　　1. Modeling material animal: Wistar rats, male, weighing (240 ± 20) g; Medications: formaldehyde, croton oil, ether; Instrument: Aseptic micro sampler.

　　2. Modeling method: Ether anesthesia is used, and the lower abdomen is opened layer by layer under sterile conditions to expose the bladder. A special stainless steel ring is used to cover the bladder, and gently pulled out to clearly expose the prostate gland (single or double lobes) in the neck after exposure. Inject the intended drug into the prostate using a sterile micro sampler. Inject 20 μ l of a mixture of 3.3 mol/L formaldehyde croton oil (8:2) into the inflammatory solution, and inject 20 μ l of sterile injection water into the control group using the same method. Remove the collar to reset the bladder and gradually close the abdomen layer by layer.

　　3. The principle of mold making applies formaldehyde Establishing a prostatitis model using a mixture of soybean oil and inflammatory solution.

　　4. Changes after modeling: The prostate weight index and total white blood cell count in the modeling group were significantly increased compared to the sham inflammation group. The prostate gland of the modeling rats showed interstitial edema, a large amount of inflammatory cell infiltration, and a small amount of inflammatory cells in the secretion inside the gland cavity, with mild degeneration and fibrosis.