动物造模,药效评价,前列腺纤维增生性炎症 z-bio 2024-11-18 447

　　1. Modeling material animal: Wistar rats, male, 250-300g; Medication: Xiaozhiling Injection, Ether.

　　2. Modeling method: The animal model was anesthetized with ether, and under sterile conditions, a midline incision was made in the lower abdomen directly into the abdominal cavity. The bladder and two seminal vesicles were extracted, and the prostate dorsal lobe attached to the inner side of the seminal vesicles was exposed. 0.2ml of 25% Xiaozhiling injection was injected and the muscle skin was sutured.

　　3. The modeling principle used Xiaozhiling injection to create a pathological model of benign prostatic fibrosis inflammation in rats.

　　4. Anatomical observation of changes after modeling: Normal prostate tissue is soft, rosy, and shiny. In the model group, 73% of the prostate tissue surface has varying degrees of adhesion and hardening with surrounding tissue, and 27% has gray white point like nodules.

　　Microscopic observation: The normal prostate cavity varies in size, with cuboidal or columnar glandular epithelium and numerous folds. The glandular cavity is filled with secretions, with less stroma and a few smooth muscle cells and fibroblasts inside. 91% of the model group showed varying degrees of lymphocyte infiltration and fibroblast proliferation in the interstitium, with interstitial widening and reduced secretion in the glandular cavity.

　　Transmission electron microscopy observation: Normal prostate cells have fewer lysosomes, abundant rough endoplasmic reticulum, enlarged into pools, neat microvilli, visible secretory granules, and well-developed Golgi complex. In the model group, microvilli disappear, secretory granules are extremely rare, and the Golgi complex is underdeveloped, with infiltration of fibroblasts, collagen fibers, and lymphocytes.