动物造模,-药效评价,慢性肾衰竭 z-bio 2024-09-24 633

　　1. Modeling material animals: rats, weighing 200-250g, half male and half female; Drug: Ether.

　　2. The modeling method involves trimming the hair at the kidney position on the back of the rat and fixing it. Under ether anesthesia, an incision was made at the left dorsal kidney position (in the sham surgery group, only the incision was made and the kidney was not cut). After fully exposing the kidney, the renal capsule was peeled off, and the upper, lower, and middle renal cortex parts were quickly removed. The incision was made in a "X" shape, and gelatin sponge was used to stop bleeding and reset the suture. One week after surgery, the right kidney was removed using the same method as described above.

　　3. The modeling principle is to establish a chronic renal failure model in rats by removing 5/6 of the kidneys.

　　4. Changes after modeling: From the fifth week after surgery, 24-hour urine volume was collected once a week. The urine volume in the modeling group increased significantly, including a significant increase in BUN, Cr, and TP content. The blood BUN and Cr content increased significantly, while TP content decreased. RBC, WBC, and Hb levels increased.

　　The systolic blood pressure of the model group significantly increased from 6 to 10 weeks after surgery, with the tail artery systolic blood pressure reaching the standard for renal vascular hypertension from 8 to 10 weeks.

　　The glomeruli exhibit moderate to severe mesangial proliferation, focal segmental glomerulosclerosis, and infiltration of tubulointerstitial mononuclear lymphocytes.