动物造模,药效评价,糖尿病 z-bio 2024-09-23 429

　　【 Spontaneous model 】 BB Wistar rat is a spontaneous diabetes animal model.

　　In 1977, Canada's Bio Breeding Institute reported a group of inbred Wistar rats with spontaneous diabetes, which can be used as animal models of insulin dependent diabetes (IDDM). In this inbred line, the incidence rate of diabetes is 30%~50%, and through breeding, the incidence rate can be increased to 90%. Most of the diseased mice are insulin-dependent, while a small number of diseased mice can survive without exogenous insulin, but show growth inhibition and chronic lesions (BB Wistar rats owned by the Institute of Experimental Animals, Chinese Academy of Medical Sciences and Shanghai Slack Experimental Animal Company).

　　【 Result Analysis 】 BB Wistar rats developed symptoms between 58-123 days of age, characterized by weight loss, hyperglycemia, diabetes, polyuria, and insulin deficiency. If insulin therapy is not administered, sick mice will experience severe ketoacidosis, dehydration, and even death in a short period of time. Autopsy results of acute onset often reveal insulinitis with lymphocyte infiltration in certain pancreatic islet areas, as well as loss and necrosis of B cell granules; The blood glucose curve shows that it is a complete diabetes type curve. The insulin secretion is at the lowest value, and there is a delay phenomenon. The shape of the pancreatic islets of the diseased animals has become irregular since the age of 20 days. The pancreatic islets of the elderly mice have become lobulated, and there is a large amount of connective tissue proliferation around, but no lymphocytic infiltration of islets has been found. Rats with acute symptoms, treated with insulin and euthanized within a few weeks after symptom onset, show a reduction in the size and quantity of their pancreatic islets, mainly composed of A cells, with very few or no B cells, and no inflammatory infiltration; The insulin content in the pancreas significantly decreases, while glucagon can be normal or reduced. No matter whether this strain of rats has diabetes or not, there is no obesity. The diabetes of BB Wistar rats is very similar to that of human insulin dependent diabetes (IDDM), but the late complications have not been determined. This strain has unique properties and is spontaneously pathogenic, which is superior to viral, chemical, or immune inducible models. It can provide a good animal model for studying the genetic, environmental, and immunological factors involved in the development of B cell damage.