动物造模,药效评价,类鼻疽杆菌 z-bio 2024-09-23 376

　　(1) The replication method selected albino guinea pigs as experimental subjects, and the bacterial suspension was inoculated intraperitoneally into the guinea pigs at a dilution of 2 × 10000000 CFU/ml. Within 96 hours after vaccination, the bacterial content in the bloodstream of bacteremia was measured every 12 hours using the petri dish culture method, and the animal mortality rate was also measured; Make pathological tissue slices, perform HE staining, and observe major organ lesions under a microscope; Observation of the ultrastructure of multi nucleated white blood cells killing Burkholderia mallei using in vitro bacteremia method.

　　(2) Within 72 hours after infection, the bacterial content in the blood of albino guinea pigs rapidly reached about 14000 CFU/ml; After 96 hours of modeling, the mortality rate of albino guinea pigs was 100%. Under microscopic histopathological observation, a large number of inflammatory cells can be seen infiltrating the main organs of the animal model. The parenchyma of the main organs is mostly accompanied by the formation of abscesses of varying sizes, proliferation of splenic lymph nodes, consolidation of lung tissue, and degeneration of liver parenchymal cells. When imitating in vitro bacteremia for 50 minutes, the bacterial structure is intact, and the number of bacteria in the PMN of albino guinea pigs is small, usually 2-3. With the extension of in vitro culture time, the bacterial structure inside the PMN of albino guinea pigs remained intact. When cultured in vitro for 100 minutes, the bacterial structure inside the PMN of albino guinea pigs was basically intact.

　　(3) Comparative medical albino guinea pigs are susceptible to Burkholderia mallei, and the method of inoculation infection is very suitable for establishing a model similar to human acute Burkholderia mallei infection. This model provides effective experimental materials for drug treatment and screening of acute Burkholderia mallei infection in clinical practice.