动物造模,药效评价,糖尿病 z-bio 2024-09-23 395

　　【 Operation steps 】 SD rats were intraperitoneally injected with a 5% pentobarbital sodium physiological saline solution at a dose of 40-50 mg/kg body weight. The rats were fixed in the dorsal position on the surgical plate, and the abdomen was shaved and disinfected with 5% iodine tincture. The surgery was performed under sterile conditions. Use scissors to make a short incision along the midline of the abdomen from the xiphoid process downwards, fully exposing the duodenal ring. Carefully peel off the pancreas with curved forceps to separate it from the mesentery of the duodenal ring; Then pull the duodenum in the opposite direction to fully expose the stomach, spleen, and colon, continue to dissect the pancreas, separate it from the pylorus and transverse colon of the spleen and stomach, and remove it. If you want animals to maintain good health, it is necessary to preserve some pancreatic tissue. After the surgery is completed, restore all organs to their normal positions. Finally, suture the peritoneum and skin incision.

　　【 Result Analysis 】 The time of clinical manifestations after surgery varies, mainly characterized by elevated blood sugar and varying degrees of symptoms such as polydipsia, polyuria, and weight loss.

　　【 Precautions 】 After surgery, wrap the animal in a towel to keep it warm, and place the animal outside the cage until fully anesthetized and put it in a dry rat cage. Within 5 days after surgery, give the animal physiological saline for drinking. Remove the stitches on the 7th day after surgery.