动物造模,药效评价,乙型肝炎 z-bio 2024-09-23 347

　　Modeling method: Ten tree shrews were used. For the first time, 0.5ml of serum (1 × 10 ^ 8 CID, chimpanzee infection dose) was intravenously injected from two asymptomatic human carriers of hepatitis B virus. Three days later, an equal amount of the same human serum was injected intraperitoneally once; PCR and in situ hybridization were used to detect HBV in serum and liver tissue at different times.

　　Result: After HBV experimental infection of tree shrews, not only can acute infection occur, but also chronic infection can be formed, with 90% showing acute infection, 56% showing chronic infection that can last for more than 1 year, and the longest lasting for 2 years and 2 months. The HBsAg positive rate in liver cells is over 80%. In the experiment of HHBV infection in tree shrews, no HBsAg was detected in the serum one week after inoculation with HHBV, indicating that there were no residual inoculants in the blood circulation of tree shrews at this time. Therefore, the HBsAg detected in the serum from two weeks after inoculation should be the replication product of HHBV in tree shrew liver cells.