动物造模,药效评价,血管性痴呆 z-bio 2024-09-23 377

　　1. Modeling material animal: SD rats, male, weighing 350-400g; Drug: Sterile naturally dried blood clots from rats of the same type, physiological saline, chloral hydrate, scopolamine.

　　2. Modeling method: Take sterile naturally dried blood clots from the same type of rats, crush them, and sieve them through a 200 μ m sieve for later use. When applied, take 0.5ml of physiological saline and 100mg of blood clot homogenate to prepare a suspension. Measure the diameter under a microscope at 40-200 μ m, with 150-200 μ m being the most common.

　　Rats were anesthetized intraperitoneally with chloral hydrate. A midline incision was made to expose the common carotid artery, which was temporarily clamped. Retrograde catheterization was performed at both external carotid arteries, and 0.5ml of saline solution was injected into the emboli. The external carotid artery was immediately ligated, and the common carotid artery was opened to allow the emboli to enter the intracranial and cerebral regions, resulting in multifocal cerebral infarction. Subcutaneous injection of 2.5mg/kg scopolamine was administered daily after surgery for one week to promote the formation of intellectual disabilities.

　　3. Modeling principle: Vascular dementia, also known as cerebral infarction dementia, is established by creating an animal model of vascular dementia through cerebral embolism.

　　4. General changes after modeling. The results of the avoidance reaction experiment showed that the learning and memory abilities of the vascular dementia rat model group were significantly lower than those of the normal control group, indicating that the intelligence of the animal model caused by this method was significantly reduced, and the animals showed certain "dementia" symptoms.

　　5. Biochemical changes after molding

　　(1) The levels of lactoperoxidase (LPO) in rats: The LPO levels in the cortex, thalamus, hippocampus, and striatum of the normal group were (74.5 ± 43.1), (98.1 ± 36.9), (34.8 ± 12.7), and (35.2 ± 12.0) nmol/100mg, respectively. The LPO levels in the cortex, thalamus, hippocampus, and striatum of the model group were (171.7 ± 45.1), (257.2 ± 46.3), (102.6 ± 23.0), and (96.3 ± 24.1) nmol/100mg, respectively.

　　(2) SOD levels in rats: The SOD levels in the cortex, thalamus, hippocampus, and striatum of the normal group rats were (32.0 ± 8.9), (13.8 ± 2.7), (3.9 ± 1.5), and (23.8 ± 4.0) ng/ml, respectively. The SOD levels in the cortex, thalamus, hippocampus, and striatum of the model group rats were (10.2 ± 7.5), (11.4 ± 3.7), (1.8 ± 1.6), and (13.1 ± 5.7) ng/ml, respectively.

　　Compared with the control group, the vascular dementia model group showed an increase in LPO and a decrease in SOD activity.

　　6. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections, surgical trauma should be minimized, and aseptic operation should be strictly enforced.