动物造模,药效评价,坐骨神经钳夹损伤 z-bio 2024-09-23 353

　　1. Modeling material animal: SD rats, male or female, 200-250g; Drug: 1% pentobarbital.

　　2. The modeling method involves surgery under intraperitoneal anesthesia with pentobarbital (0.4mg/kg) and sterile conditions. A longitudinal incision was made on the lateral side of the left lower limb of the animal, and a specially designed vascular clamp was used to clamp the sciatic nerve at the intersection of the line connecting the greater trochanter of the ischium and the ischial tuberosity for 15 seconds, with the disappearance of the distal action potential as the criterion for complete injury.

　　3. The principle of modeling is to clamp the sciatic nerve of rats to form a nerve injury model.

　　4. Changes after modeling: After being clamped, the action potential of the sciatic nerve in rats cannot pass down from the site of injury, resulting in temporary loss of physiological function. After nerve trunk injury, the action potential is completely or partially blocked, and as the injury is repaired, the action potential gradually recovers.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections, surgical trauma should be minimized, and aseptic operation should be strictly enforced.