动物造模,药效评价,心肌病 z-bio 2024-09-23 306

　　1. Experimental animal: Wistar rats, two weeks old, male or female.

　　2. Experimental method: Wistar rats were treated with 700 × 10 ^ -6 furazolidone added to drinking water (700mg furazolidone per kilogram of water) and observed for 12 weeks.

　　3. Experimental observation content determination method: Ultrasound monitoring of cardiac function, measuring the M2 curve of left ventricular long axis section and left ventricular papillary muscle level, continuously measuring the left ventricular end diastolic diameter (LVDD, mm), left ventricular end systolic diameter (LVSD, mm), left ventricular end diastolic volume (LVDV), and left ventricular end systolic volume (LVSV) for three cardiac cycles, taking their average values, and converting the left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) using Simpson's method. The calculation formula is LVEF=[(LVDV-LVSV)/LVDV] × 100%, LVFS=[(LVDD-LVSD)/LVDD] × 100%. As an indicator of cardiac function parameters.

　　4. Referring to the results, the mortality rate of rats after 12 weeks of modeling was 10%, and the success rate of modeling was 66.6%. In the DCM group, the myocardial cells of each subgroup of rats showed proliferation and hypertrophy, focal dissolution of myocardial cells, varying degrees of granular and vacuolar degeneration, nuclear enlargement, partial division and malformation, widened intercellular gaps, and normal intercellular gaps. Research has found that feeding with furazolidone for 8 and 12 weeks does not increase the incidence of furazolidone dilated cardiomyopathy. It is speculated that the cardiotoxicity of furazolidone is age-related, so furazolidone should be administered as early as possible, preferably one week after birth in rats.