动物造模,药效评价,心肌缺血 z-bio 2024-09-23 311

　　1. Modeling material animals: healthy dogs, weighing 10-20kg; Medications: pentobarbital, penicillin; Equipment: airbag compressor, animal artificial ventilator.

　　2. Modeling method: After anesthetizing the animal, artificial respiration is performed. After opening the chest from the left 4th or 5th intercostal space, the left anterior descending branch of the coronary artery is separated by about 1cm (or a segment of the left circumflex branch), and placed in a compression device. When the balloon of the compression device is inflated, it compresses the blood vessel and blocks blood flow, causing short-term myocardial ischemia and long-term myocardial infarction.

　　Suture 11-15 electrodes or fix multi-point epicardial electrodes on the pericardium, connect a multi-channel physiological recorder, electrode wires, and plastic catheters of the compression device through the chest wall and lead them out of the body. Suture the chest wall layer by layer, and expel the chest gas. After surgery, inject penicillin intramuscularly for 3 days, and put a vest on the animal to protect the wound, catheter, and wires. After chest closure, dogs can still block coronary artery blood flow under anesthesia to observe ischemic reactions under anesthesia. After recovery, dogs can observe the electrocardiogram of the pericardium after coronary artery occlusion under clear and quiet conditions. The duration of each coronary artery occlusion is 3-5 minutes, and the interval between secondary occlusions is 20-30 minutes. Approximately 2-5 occlusions are performed per day, and continuous observation can be conducted for 6-10 days.

　　3. The principle of modeling is that the compression device intermittently blocks the blood flow of the animal's coronary artery, leading to myocardial ischemia in the animal.

　　4. Changes after modeling: The electrocardiogram of the animal showed myocardial ischemia after modeling.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections, surgical trauma should be minimized, and aseptic operation should be strictly enforced.