动物造模,药效评价 z-bio 2024-09-23 352

　　Trichomoniasis is a zoonotic parasitic disease that can host humans, pigs, mice, cats, dogs, and various wild animals. The adult and larvae of Trichinella spiralis parasitize in the same host, with the adult parasitizing in the small intestine and the larvae parasitizing in the permanent muscle cells. Trichinella causes a series of damages to its host during development, migration, and parasitism. Guo E and others conducted experimental research on mouse trichinosis, observing the clinical symptoms and pathological changes of experimental rats; Zhu Xingquan et al. conducted a study on the natural resistance of mice to trichinella infection and believed that mice are suitable model animals for large-scale experimental research on trichomoniasis; Liu Hui et al. conducted experimental research on the infection of Trichomonas reinhardtii in Mongolian long clawed sand rats and believed that Mongolian long clawed sand rats are ideal animals for establishing a model of Trichomonas reinhardtii for parasitic and drug research, while mice are more suitable for the conservation of Trichomonas reinhardtii.

　　(1) Method of replication: ① Select long clawed sand rats weighing 45-61g and aged 9-12 weeks. ② Count the muscle tablets of the host containing the larvae of Trichinella spiralis Feed 80 long clawed sand rats per animal.

　　(2) The clinical symptoms, mortality, or survival status of sand rats infected with Trichinella larvae are related to the amount of infection. After about 20 days of infection, there will be chills, wet fur, reduced food intake, and inability to stand. When the infection is severe, there may be conjunctival congestion, edema, and excessive secretion. After 50 days of infection, the larvae gradually recovered, but showed significant weight loss, regression, and atrophy of the abdominal wall muscles, and the larvae inside the muscles had become cysts. This model is very similar to the onset time and clinical manifestations of human larvae during the migration and cyst formation stages after infection with Trichinella spiralis.

　　(3) The clinical symptoms, time of death or survival status after comparative medical infection with Trichinella larvae are not only related to different species of animals, but also to the amount of infection. The model replicated by this method can be used for teaching, scientific research, drug research, and other applications of Trichinella spiralis.