动物造模,药效评价,高血压 z-bio 2024-09-19 301

　　Repeated combination of unconditional stimuli (such as injection of adrenaline, angiotensin, or electrical stimulation) with conditioned stimuli can induce hypertension in animals such as dogs, rats, and rabbits.

　　(1) Method of replication: Male Wistar rats at 12 weeks of age were subjected to stress stimulation before the experiment. The model animals were selected as those who exhibited rapid avoidance, screaming, tail raising, and wheezing, which were difficult to adapt to. Select a composite stimulation of plantar electric shock with randomly changing stimulation pulses and noise. The intensity of the stimulation pulse power supply should not cause damage, but should cause strong reactions. The current is 0.1-0.8mA, the pulse interval is 20-150ms, the wave width is 2-20ms, and the stimulation voltage output is 75-150V (adjustable). The frame and walls of the experimental mouse box are made of organic glass plates, measuring 22cm × 22cm × 16cm. The bottom of the box is lined with 3mm diameter copper tubes in a grid shape, with a spacing of 1cm between the tubes and connected to a stress source. The noise source is placed in front of the stress chamber and not in contact with the mouse chamber. The experimental group of rats received stress stimulation once a day from 8:00 to 10:00 in the morning and from 2:00 to 4:00 in the afternoon, for a total of 15 days. Measure blood pressure and heart rate using tail cuff method on the first day before applying stress stimulation and 0.5 hours after stress stimulation on the 3rd, 6th, 9th, 12th, and 15th days, and observe their behavioral changes. Prepare models with different stress times, with stress cycles of 3, 6, 9, 12, and 15 days. During the stress process, the blood pressure of rats significantly increased, rapidly rising to (156.4 ± 14.0) mmHg (1mmHg=0.133 kPa) within 6 days, which was the highest value of blood pressure after stress. On the 9th day, it was (150.5 ± 12.0) mmHg. Afterwards, it remained relatively stable at this high level and remained at (152.4 ± 11.0) mmHg until the 15th day. The heart rate significantly increased from (406.0 ± 18.0) beats/min to (434.6 ± 20.0) beats/min on the third day after stress, and increased to (478 ± 21.0) beats/min on the sixth day, reaching (463.0 ± 17.0) beats/min on the fifteenth day.

　　(2) Model characteristics The incidence rate of hypertension animal model induced by stress using this method is higher than that of the same type of experimental stressors, and the time required for stress stimulation is short. The experimental stressors used are superior to the same type of stressors.

　　(3) Comparative medicine uses electric shock and random noise signals as conditioned stimuli, greatly increasing the psychological component of stress and facilitating animal psychological reactions, causing hypertension in animals. It has similarities with human stress-induced hypertension disease, is reproducible, and the program can be quantified.