动物造模,药效评价,动脉粥样硬化 z-bio 2024-09-19 401

　　(1) The replication method involves male quails weighing 90-110g and aged 5-6 weeks. 1% cholesterol, 1% lard and 1% soybean oil were mixed in the feed. After 6 weeks, the serum cholesterol content was significantly increased, and aortic plaque appeared. The aortic intima was slightly changed. Microscopically, a few foam cells were scattered. After 16 weeks, the aortic intima was significantly thickened, with obvious patches. Microscopically, a large number of foam cells were seen, and the intima was significantly thickened. At 32-36 weeks, the intima was significantly thickened, fibrous hyperplasia, cell necrosis, and necrosis areas were found. Increasing the proportion of feed fat can accelerate atherosclerosis. If it is reported that the feed containing 14% lard, 6% peanut oil and 1% cholesterol can form atherosclerosis plaques after 6-8 weeks of feeding.

　　(2) Model features: This model is modeled using quails, with a simple method and high modeling rate. But after 32-36 weeks of high-fat feeding and 4-6 weeks of basic feed feeding, the thickness of the aortic intima and plaque score were significantly reduced compared to when high-fat feed was stopped, indicating that As may naturally recover after stopping high-fat feed. At the same time, there is a certain correlation between modeling time and disease severity. At the same time, due to the small size of individual animals, the consumption of experimental drugs is relatively low, making feeding, management, blood collection, and administration convenient.

　　(3) The aortic lesions in quails, a comparative medical model animal, are similar to early human fat spots, with severe lesions at the bifurcation of the arteries and limited to the intima. The experiment induced As plaques in quails with 1% cholesterol and 2% oil, and the severity of the pathological morphological changes of As plaques was closely related to the formation time. At 16 weeks, the arterial intima plaques were raised, the intima was significantly thickened, the number of smooth muscle cells decreased, and the nuclei were stained and condensed. The pathological morphological changes were similar to those in humans.