动物造模,药效评价,肾小球硬化 z-bio 2024-09-19 444

　　1. Modeling material animals: Male SD rats, 3 months old, weighing 220-250g; Medications: Anesthetics, Antibiotics, Doxorubicin.

　　2. Modeling method: After one week of adaptation, the animal underwent right nephrectomy. On the 7th day after surgery, 2g/L of doxorubicin solution was injected into the tail vein at a dose of 5mg/kg. On the 30th day after surgery, doxorubicin was repeatedly injected at a dose of 3mg/kg.

　　3. The modeling principle is to establish a chronic renal failure rat glomerulosclerosis model by right nephrectomy and tail vein injection of doxorubicin in SD rats.

　　4. Changes after modeling: The postoperative status of the modeling rats was good. On the 7th day, doxorubicin (5mg/kg) was injected into the tail vein, and there were no adverse reactions such as diarrhea after medication. On the 20th day of medication, edema appeared, and during the 12th week of modeling, it was found that the model group rats were significantly emaciated and had ascites.

　　The model group rats showed proteinuria, which began to increase in urine protein in the second week, reached its peak in the fourth week, and slightly decreased from the eighth week onwards. At 12 weeks after right nephrectomy, the serum albumin of the model group rats was significantly lower than that of the control group, while cholesterol (CHO), urea (Ure), and Scr were significantly higher than those of the control group. In the second week, the NO2- content in the model group began to decrease and reached its lowest point in the eighth week, while ET-1 began to increase in the second week and reached its peak in the eighth week.

　　Under light microscopy, the model group showed mild mesangial cell proliferation, diffuse moderate proliferation of the matrix, frequent balloon adhesions, moderate or severe thickening of the balloon wall, nodular dilation of capillary loops, moderate fibrosis of the interstitium, sheet-like dilation of small tubes, and frequent protein casts. Testing showed successful modeling of glomerulosclerosis. Under electron microscopy, the foot processes of the glomerular epithelial cells in the model group were extensively fused or even disappeared, with mild proliferation of mesangial cells, moderate proliferation of matrix, and moderate or severe thickening of the glomerular capillary basement membrane (GBM).