动物造模,药效评价,牙髓炎 z-bio 2024-09-18 413

　　1. Experimental method: There are 5 Chinese experimental miniature pigs weighing 14-26kg, with 3 males and 2 females. Select the first permanent molars of the upper and lower jaws, and create a pulpitis model with bilateral mandibular incisors. After anesthesia with compound thiamethoxam at a dose of 0.1 ml/kg via intramuscular injection behind the ear in small pigs, thoroughly clean the oral area and mouth. Disinfect the experimental teeth and periodontal tissue with 2% iodine tincture, use a high-speed turbine drill with a complete cooling device to prepare holes on the surface of the experimental molars, and prepare V-shaped holes for the mandibular incisors. Carefully penetrate the pulp with a diameter of 2-3mm, rinse repeatedly with physiological saline, and fully stop bleeding. The control group used calcium hydroxide paste to cover the pulp, zinc oxide clove oil paste as the base, and zinc phosphate cement for filling. The experimental group directly covered the pulp with zinc oxide clove oil paste and filled it with zinc phosphate cement. Strict aseptic operation during surgery. Animals were euthanized at 2 weeks, 4 weeks, and 3 months after surgery, and experimental and control teeth were quickly removed, numbered, and fixed in 10% formalin. Histopathological observation: Microwave decalcification using a mixture of hydrochloric acid, formic acid, and sodium chloride, gradient alcohol dehydration, and continuous sectioning with conventional paraffin embedding, with a thickness of 5 μ m. HE staining, observed under a light microscope.

　　2. Experimental results: Two weeks after the calcium hydroxide pulp capping surgery in the control group, a thin layer of pulp necrosis was observed below the perforation hole, accompanied by fibroblast proliferation and occasional formation of a small amount of calcified masses. Square shaped odontoblasts were found around the pulp, and there was significant infiltration of inflammatory cells in the pulp. There was also a small amount of blood vessel proliferation and congestion in the tissue. One month after pulp capping surgery, a reparative dentin mass can be seen forming in the teeth, with a dense structure and tubular dentin. There are columnar odontoblasts around, but no complete dentin bridge is formed. The pulp below the reparative dentin is congested with a small amount of inflammatory cells. Three months after pulp capping surgery, a complete reparative dentin bridge can be seen, with irregular shape and tubular dentin, tightly integrated with the primary dentin, and normal pulp.

　　Two weeks after the pulp capping surgery with zinc oxide clove oil paste in the experimental group, there was significant infiltration of inflammatory cells below the pulp hole, vascular dilation, partial pulp necrosis, and no reparative dentin formation. One month after surgery, a small amount of irregular calcified masses can be seen in the medullary cavity, surrounded by odontoid like cells, without complete dentin bridge formation. Three months after surgery, reparative dentin was formed with irregular morphology, surrounded by odontogenic cells, and the medullary foramen was not completely closed.