动物造模,药效评价,急性肾衰竭 z-bio 2024-09-13 504

　　1. Modeling material animals: Adult male rats weighing 200-300g; Medications: Anesthetic, inulin, Escherichia coli lipopolysaccharide (LPS).

　　2. Modeling method: Place the animal under anesthesia on a warm operating table, with a basal body temperature maintained at 37-37.5 ℃. Cut open the trachea, insert a tracheal tube, cut open the skin in the groin area, separate the femoral artery and vein, and inject 7% inulin solution into the jugular vein mixed with physiological saline at a rate of 1.2ml/h. Expose the left kidney and left ureter through a left intercostal incision, insert a ureteral catheter, and connect the left renal artery to an electromagnetic flowmeter probe to display changes in renal blood flow; Dissolve Escherichia coli lipopolysaccharide (LPS) in distilled water and store at 4 ℃. Each rat was intravenously injected with LPS 100mg/kg, and the injection was completed within 3 minutes.

　　3. Modeling principle: Lipopolysaccharides cause acute renal failure in animals.

　　4. After modeling, the changes in various indicators were observed at 35-50 and 70 minutes after injection of LPS. The results showed an increase in body temperature, a decrease in renal blood flow and glomerular filtration rate. The renal tissue structure after injection of LPS was roughly similar to that of normal rats, with no increase in glomerular epithelial cells and no deposition of blood platelets in the vascular endothelium.