动物造模,药效评价,急性肾衰竭 z-bio 2024-09-06 583

　　1. Modeling material animals: Male SD rats weighing 200-300g; Drug: Cisplatin.

　　2. Modeling method: Animals were adaptively fed for one week before use in the experiment. Cisplatin was diluted to 2mg/ml with distilled water before the experiment. Inject 6mg/kg cisplatin (2mg/ml distilled water) via the tail vein of rats or dissolve 10mg/kg cisplatin in 1ml physiological saline for a single intraperitoneal injection.

　　3. Modeling principle: Cisplatin (CP) has certain toxicity in clinical applications and can be used to establish ARF models.

　　4. Changes after modeling: On the fourth day (72 hours) after medication, Scr and BUN significantly increased, about three times the baseline values. On the 10th day, although Scr decreased slightly, it was still twice the baseline value.

　　Pathological observation of renal tissue: There were no significant histological changes in the control group. The pathological changes on the 6th and 10th days of the model group were basically similar. There is no significant change in the glomeruli. Most of the epithelial cells in the renal tubules of the extrarenal medulla undergo necrosis and shedding. The nucleus shrinks, dissolves, and disappears, the lumen expands, and there are shedding debris.