动物造模,药效评价,球囊损伤 z-bio 2024-09-05 320

　　(1) The replication method involves immobilizing the rabbit under conventional anesthesia and separating one side of the femoral artery and vein. 20ml of blood was collected through femoral vein catheterization, and platelets were labeled with 51Cr. After injecting marked platelets into the ear vein, immediately insert a balloon catheter through the femoral vein. When the catheter tip reaches the junction of the thoracic and abdominal aorta, inflate the balloon to 600mmHg (80kPa), pull down the balloon catheter 10cm, deflate it, reinsert it at the junction, inflate again, pull down, repeat this process 6 times, remove the catheter, ligate the artery, and suture the incision. Animals were euthanized by intravenous injection of excessive pentobarbital sodium 1 hour after endometrial removal. Before execution, administer 5ml of 0.5% Evans blue and 1000U of heparin intravenously. Separate the aorta, cut the upper 5cm thoracic aorta and the lower 10cm uniformly blue stained abdominal aorta, rinse twice in physiological saline, count the CPM value of each arterial segment, fully dry and weigh, and calculate the number of platelets marked on each gram of dry weight arterial segment.

　　(2) The model features the use of balloon catheter injury to the endometrium, forming an abdominal aortic thrombus model in vivo in rabbits. The method is reliable, simple, and easy to implement, with advantages such as low subjective factors and quantitative analysis.

　　(3) When comparing medical balloon catheter injuries to create arterial thrombosis, the intima is also damaged to varying degrees. In clinical practice, arterial thrombosis formed by intimal injury is limited to the surface and is more severe than clinical injury. However, the nature of the thrombus formed by intimal injury is consistent with that of arterial thrombosis formed by clinical intimal injury, and does not affect the observation of drug efficacy.