动物造模,药效评价,弥散性血管内凝血 z-bio 2024-09-05 402

　　(1) Preparation of rabbit brain powder infusion: The rabbit is euthanized by bloodletting, and the attached blood vessels and meninges are removed. After rinsing, the rabbit brain is dried with gauze and placed in a mortar with acetone. The brain tissue is ground and crushed for about 5 minutes each time, and the acetone is poured out. The acetone is repeatedly replaced and ground 6-7 times to remove water and fat until the brain is ground into gray white particles. Finally, the particles are spread flat on filter paper and placed in a 37 ℃ incubator for 0.5 hours. After the acetone evaporates, the brain tissue becomes powder and placed in a small test tube, sealed, and stored at -20 ℃ for later use. Modeling method: Take 2.5-3.0kg experimental rabbits, fix them in a supine position after anesthesia, disinfect and remove hair from the surgical area of the neck, separate the tissue layer by layer during surgery, and perform carotid artery catheterization. Inject 4.5ml of blood from the carotid artery into a centrifuge tube containing 0.5ml of 3.8% sodium citrate solution, and mix immediately. Add 5-10ml of sterile physiological saline solution from the ear vein, and inject 2% rabbit brain powder infusion (3ml/kg) intravenously at a rate of around 2ml/min. 20 minutes after injection of rabbit brain infusion, open the carotid clip and perform a second bloodletting. Centrifuge the blood obtained twice before and after at 3000r/min for 5 minutes, separate the plasma for quantitative measurement of fibrinogen, determination of kaolin partial thromboplastin time (KPTT) and prothrombin time (PT), and conduct a plasma prothrombin coagulation test (3P).

　　(2) After 20 minutes of injection of rabbit brain infusion, there were significant differences in various indicators between the model animals and the normal control animals. The fibrinogen content decreased significantly compared to before injection. The white clay partial thromboplastin time of plasma reflecting endogenous coagulation system activity is prolonged, and the plasma prothrombin time reflecting exogenous coagulation system activity is prolonged; The index for determining soluble complexes of fibrinogen monomers in plasma - plasma protamine coagulation test, negative before injection and positive after injection. In addition, platelet count was significantly reduced during DIC.

　　(3) Comparative medicine compares and analyzes the data and results obtained from replicating rabbit DIC models with human disease manifestations, which can be used to elucidate the mechanism of DIC occurrence, reveal the laws of disease development, and provide theoretical basis for clinical disease diagnosis and treatment.