动物造模,药效评价,肾衰竭 z-bio 2024-09-05 461

　　1. Modeling material animals: rats, weighing 220-250g; Drug: Oleic acid.

　　2. Modeling method: Rats were randomly divided into 5 groups: physiological saline control group, oleic acid groups I, II, III, and IV: 0.45ml of physiological saline, 0.5ml/kg, 0.1ml/kg, 0.125ml/kg, and 0.15ml/kg of oleic acid were injected into the left renal artery of rats, respectively.

　　3. The principle of modeling is based on the ARF model established using oleic acid.

　　4. Changes after modeling: Injection of oleic acid causes swelling and necrosis of endothelial cells in the glomeruli and capillaries surrounding the renal tubules, necrosis of the renal tubules, and an increase in BUN. In addition, the degree of damage to renal tissue structure increases with the increase of oleic acid dosage.