动物造模,药效评价,急性肾衰竭 z-bio 2024-09-04 429

　　1. Modeling material animals: male domestic dogs, weighing 10-20kg; Medication; Pentobarbital, norepinephrine.

　　2. Modeling method: After anesthesia with intravenous injection of 30mg/kg pentobarbital, one side of the ureter was surgically separated and a catheter was inserted to collect urine. Inject norepinephrine into the renal artery at a rate of 0.75 μ g/kg per minute for 40 minutes, then discontinue the medication. The experiment can be conducted within 1-4 hours.

　　3. Modeling principle: Norepinephrine causes renal vasoconstriction, resulting in reduced blood flow in the glomerulus and decreased glomerular filtration; Renal tissue ischemia and hypoxia exacerbate damage to renal tissue, leading to ARF.

　　4. Changes after modeling: After medication, renal blood flow sharply decreased and ipsilateral ureteral urine volume decreased.

　　5. Precautions: The urine collection tube should be filled with warm water in advance to prevent the tube from bending and obstructing urine flow: Do not give a high protein diet before the experiment to avoid affecting BUN levels.