动物造模,药效评价,糖尿病视网膜病变 z-bio 2024-09-03 545

　　[Modeling mechanism] NOD mice are the best model for studying human type 1 diabetes, and they are very similar to humans in genetics and immunity. The main susceptibility gene of human type 1 diabetes is located in MHC, and the susceptibility gene of NOD mice is also located in MHC.

　　【 Model Characteristics 】 NOD mice develop pancreatitis at 4-5 weeks, followed by subclinical B cell destruction and decreased insulin concentration in the bloodstream. The typical symptoms of diabetes occur in 12 to 30 weeks, including hyperinsulinemia, hyperglycemia, ketoacidosis and death caused by autoimmune damage of lymphocyte infiltration and destruction of islet B cells. There is gender difference in the occurrence of diabetes in NOD mice. 90% of female mice have diabetes, and only 20% of male mice have diabetes. At 6 weeks, the expression of hypoxia inducible factor-1 alpha (HIF-1 alpha) in the retina was lower, while at 12 weeks, the expression of HIF-1 alpha in the retina was significantly higher than that in the control group. Microvascular disorder and local proliferative neovascularization may occur within 8 months. Angiotensin II and thromboxane mediate small vessel constriction.

　　[Model source] Jackson laboratory in the United States can provide NOD diabetes mice.

　　[Model evaluation and application] NOD mice are one of the animal models of spontaneous diabetes, which provide valuable information for the study of the etiology of type 1 diabetes and hereditary diabetes retinal disease, and have valuable value in the study of immune regulation and immune tolerance.