动物造模,药效评价 z-bio 2024-08-28 496

　　The principle of platform sleep deprivation is to utilize the living habits of rats, which are afraid of water and unable to enter sleep in water. By placing a platform in a water tank, rats can stand on the platform. Due to the small diameter of the platform (≤ 6.5cm), when rats enter a sleep state, their whole body muscle tension decreases, causing them to rhythmically lower their heads (touch water), in order to achieve the goal of sleep deprivation. This model is simple and easy to implement, does not require complex and expensive equipment, and can be carried out in laboratories under different conditions, making it widely used. Both male and female rats can be used, with a body weight of 180-220g.

　　Single platform sleep deprivation method

　　(1) Copy method: Place a cylindrical small platform (with a diameter of about 6.5cm) in a 40cm × 34cm × 16cm water tank, and add water to the tank until the platform is about 1cm above the water surface. Place one rat on a small platform, and the rat can stand on the platform and enter non rapid eye movement sleep (NREM). When the rat enters rapid eye movement sleep (REM), the tension of its skeletal muscles decreases significantly, and the tension of its neck muscles decreases rhythmically, causing it to lower its head (touch water) and unable to enter REM.

　　(2) The use of SP experiments can cause a decrease in animal body weight and thymus weight, an increase in adrenal gland weight, an increase in cortisol (CORT) in plasma, an increase in animal aggressive behavior, and suppression of the body's immune response. This model still has shortcomings such as the separation of rats from the population and limited activity space.

　　2 Multi platform Sleep Deprivation Methods

　　(1) In order to expand the activity space of rats in MP, the replication method increased the number of small platforms from one in SP to seven, while still placing one rat between the small platforms for sleep deprivation experiments. Due to the fact that rats can freely move between 7 small platforms, their range of activity has significantly increased compared to SP.

　　(2) This method is developed on the basis of SP to overcome the limitation of activity space in SP. After experimental comparison, some indicators that reflect stress enhancement in MP, such as increased adrenal gland weight and decreased thymus weight, still exist, and even the increase in ACTH, CORT, and adrenal gland weight in rat blood is higher than that in the SP model, indicating that MP has stronger stress response than SP.

　　3 Improved Multi Platform Sleep Deprivation Methods

　　(1) The replication method is further improved on the MP method to overcome the stress response caused by group isolation. In MMP, 10 rats (the specific number can be determined according to actual needs) were placed simultaneously in a water tank with 14-15 small platforms (127cm × 44cm × 45cm) for the experiment. This not only avoids the disadvantage of isolating a single rat from the population in SP and MP, but also retains the characteristic of increased activity range in MP. If the 10 rats required for the sleep deprivation experiment are placed in the same cage for 2 weeks before the experiment, and then these rats are placed together for the sleep deprivation experiment, this can overcome the instability of the rat population during the experiment and minimize the uncertainty factors during the experiment.

　　(2) After taking measures to maintain the stability of the rat population and avoid separation from the population in MMP, the adrenal gland weight, weight loss, and serum levels of adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in rats were all lower than those in MP, indicating that MMP has lower stress levels than MP and is an ideal sleep deprivation method.