动物造模,药效评价,犬肾病 z-bio 2024-08-28 390

　　1. Modeling material animal: dog; Drug: mercuric chloride.

　　2. Modeling method: Before the experiment, the dog is first subjected to ureteral fistula or bladder fistula, and a 2% mercuric solution is injected subcutaneously into the abdomen of the modeling animal at a dose of 20mg/kg.

　　3. Modeling principle: Mercury poisoning can cause kidney disease in dogs.

　　4. Changes after modeling: 1-2 hours after modeling, the urine volume increases sharply, which can be 5-10 times the original urine volume, and the specific gravity decreases from 1.007 to 1.001. After a few hours, the urine volume begins to decrease, and on the second day, it develops into oliguria or anuria, with a specific gravity increasing to 1.045-1.049 and a small amount of protein. At the same time, it shows general symptoms of mercury poisoning, such as vomiting, salivation, bloody stools, refusal to eat, and excessive water intake. Death occurs on the 3rd to 10th day after poisoning. After dissection, it was found that the volume of the kidney had increased, and the tissue appeared slightly reddish brown with numerous bleeding points. There was also bleeding in the gastrointestinal tract.