动物造模,药效评价,氟斑牙 z-bio 2024-08-27 437

　　(1) The replication method involves selecting female rats weighing 70-100g after weaning, and preparing different concentrations of sodium fluoride solution for poisoning with distilled water. The poisoning volume is calculated at 0.5ml/100g body weight. At a dose of 50mg/kg body weight, animals were orally administered sodium fluoride solution on an empty stomach every day. After 1 hour of gavage exposure, the animals were fed. The model animals were fed normally, with free access to water (tap water had a fluoride content of 0.62mg/L) and food (feed had a fluoride content of 0.84mg/kg body weight). Each animal was exposed to the same dosage and method for 6 days per week, and observed continuously for 2 months.

　　(2) On the 20th to 25th day of oral administration of sodium fluoride solution, all model animals developed significant fluorosis teeth. Most of the brown striations on the surface of the teeth have disappeared, and the entire tooth surface appears as a white chalk with a tooth margin defect. The changes in enamel glossiness, enamel staining, and enamel defects are all pathological changes similar to the clinical manifestations of human fluorosis. This model has a simple production method, low cost, and good repeatability.

　　(3) The comparative medicine model was developed by administering a high fluoride solution to animals, which is consistent with the natural causes of fluorosis in humans. The clinical manifestations and pathological changes of fluorosis in the model rats are extremely similar to those in humans in terms of enamel glossiness, staining, and defects. Therefore, this model is suitable for studying the pathogenesis, prevention, and control of fluorosis in humans. At the same time, the production process and results of this model show that the dose of fluoride exposure is closely related to the incidence of dental fluorosis and the formation time of dental fluorosis. The greater the dose of fluoride exposure, the shorter the formation time of dental fluorosis, and the higher the incidence rate.