动物造模,药效评价,血清病肾炎 z-bio 2024-08-27 316

　　1. Modeling material animal: rabbit; Drug: Bovine serum albumin (BSA).

　　2. Modeling method: BSA was administered intravenously once to rabbits at a dose of 250mg/kg. On days 6, 8, 10, 13, 15, 17, and 20 after injection, urinary catheterization was performed to check for protein, and blood was collected to measure circulating antigen antibody complexes (CIC). After the last blood collection, the animals were euthanized and underwent kidney disease testing.

　　3. Modeling principle: Induction of animal glomerulonephritis model by heterologous immune complexes.

　　4. Changes after modeling: After modeling, the CIC values of rabbits were all higher than normal. The onset time of nephritis is 7-11 days or 13-14 days, and the changes in urinary protein are consistent with histological changes. Under the microscope, typical changes of acute proliferative nephritis are observed, with an increase in glomerular volume, an increase in the number of capillary plexus cells, proliferation of endothelial and stromal cells, and infiltration of neutrophils. Fluorescence microscopy shows that glomerular capillaries are arranged with particles of varying sizes from the basement membrane.