动物造模,药效评价,急性胃炎 z-bio 2024-08-26 376

　　(1) Method of replication: Adult male rats were fasted without water for 48 hours, orally administered with 100mg/kg body weight of acetylsalicylic acid, or 10mmol/L acetic acid or different concentrations of hydrochloric acid (1, 10, 10mmol/L), or administered with the same bile or 2mmol/L taurocholic acid or 15% ethanol alone or in combination. After 4 hours, the rats were euthanized, and the entire stomach was immediately cut open and inverted along the greater curvature of the stomach. The contents were poured out, and the gastric residue was gently washed with cold physiological saline. The mucosal lesions were observed, and histopathological examination was performed using light and electron microscopy techniques.

　　(2) The common pathological feature of model animals is acute diffuse inflammation of the gastric mucosa. If a mixture of hydrochloric acid and acetylsalicylic acid is administered by gavage, the gastric mucosa of the model rats shows congestion and edema. In severe cases, there are patchy bleeding points and diffuse erosions. Under light microscopy, epithelial cells can be seen to have varying degrees of degeneration and shedding, neutrophil infiltration in the lamina propria and submucosal layer, and interstitial congestion and edema.

　　(3) Acute gastritis in comparative medicine can be caused by physical, chemical factors, and microbial infections. Physical factors commonly include overeating or consuming food that is too cold, too hot, or too rough, leading to congestion, edema, and sometimes erosion of the gastric mucosa; Chemical factors are mostly caused by taking certain nonsteroidal anti-inflammatory drugs such as salicylic acid preparations, certain hormones, high concentrations of ethanol, or swallowing strong acids, strong bases, or other corrosive liquids such as hydrochloric acid and acetic acid, which can lead to gastric mucosal bleeding, necrosis, and dissolution. In severe cases, it can involve deep tissues or even perforation; Microbial infection refers to the consumption of food contaminated with microorganisms and bacterial toxins, or direct infection caused by gastric trauma. This model uses the above-mentioned pathogenic factors to induce acute gastritis in animals, and its pathological characteristics are similar to clinical features. The method is simple and the success rate of modeling is high, which has been widely used.