动物造模,药效评价 z-bio 2024-08-22 500

　　(1) The replication method uses male adult rats weighing 250-270g. Abdominal infection group underwent intestinal fistula surgery: Rats were fasted for 8 hours before surgery, anesthetized with ketamine injected intraperitoneally at a dose of 100mg/kg body weight, and then fixed supine on the surgical plate; Shave the abdominal surgical area, disinfect with iodine, make a midline incision in the lower abdomen, cut off one-third of the circumference of the small intestine 20cm away from the ileocecal area, and suspend the mesentery edge of the intestinal wall on the right abdominal wall with 3-0 suture at a distance of 1cm above and below the perforation site. The first suture is divided into two layers to close the abdomen. The model animals were euthanized 48 hours after modeling, and peripheral blood white blood cell counts were performed. At the same time, peripheral blood and peritoneal fluid were cultured for 48 hours in blood culture dishes, and colony counting and strain identification were performed; Cut the intestinal segment with fistula 2cm above and below the fistula opening in the model animal, perform routine tissue sectioning, HE staining, and light microscopy examination.

　　(2) Model characteristics: The peripheral blood WBC of the model animals began to increase 12 hours after surgery, and the peritoneal fluid culture mainly consisted of Escherichia coli and Group D streptococcus; After 16 hours, the model animal may die, and the intestinal tract of the animal is visibly dilated and congested with edema. The intestinal wall is brittle and prone to bleeding. Under the light microscope, it can be seen that the serosal layer, muscular layer, and intrinsic layer of small intestine villi are all significantly congested, accompanied by infiltration of inflammatory cells. The mesenteric blood vessels are dilated and congested, and there is infiltration of inflammatory cells around the blood vessels. Compared with models replicated by other methods, the abdominal infection model replicated by this method has a lower mortality rate, relatively good stability, and is suitable for repeat intestinal surgery.

　　(3) The comparative medicine model can simulate the symptoms of abdominal infection in clinical patients and has typical manifestations of peritonitis; And the production method is simple, the results are stable and reliable, and it is easy to perform intestinal surgery again. When using this model for experimental research and observation, it can also achieve the purpose of simulating clinical intestinal fistula, and has good practical value.