动物造模,药效评价,缺血再灌注肝损伤 z-bio 2024-08-22 412

　　(1) Male KM mice weighing 18-22g were subjected to intraperitoneal injection of pentobarbital sodium anesthesia at a dose of 30mg/kg body weight. After anesthesia, the animals were fixed in a supine position on a surgical plate, and the abdominal surgical area was routinely disinfected and depilated; Cut along the midline of the abdomen, expose the abdominal cavity, separate the superior mesenteric artery, and clamp its root with non-invasive arterial clips to induce ischemia. Control the ischemia time for 20 minutes; Then release the arterial clamp to restore smooth blood flow, and perform reperfusion for 1 hour. After 1 hour, collect mouse blood (prepare serum) and euthanize the animal. After animal necropsy, take the liver for routine pathological tissue sections.

　　(2) The model features a mouse mesenteric artery ischemia-reperfusion liver injury model made using this method. During ischemia, the activity of superoxide dismutase (SOD) in the model animals decreased, while the content of malondialdehyde (MDA) in the liver showed an increasing trend. The changes in SOD activity and malondialdehyde content in vivo increased with ischemia time; When the mesenteric artery is ischemic for 20 minutes and reperfusion for 1 hour, the activities of serum alanine transaminase (ALT) and glutathione S-transferase (GST) in mice can be significantly increased. Under the microscope, the structure of liver lobules in the liver tissue of the model animal is still visible, but the liver cells show watery degeneration, and the cytoplasm is loose and vacuolated; Some liver cell nuclei are heavily stained and have undergone consolidation and reduced volume; Small focal necrosis can be seen in the liver tissue, and the structure of liver cells in the necrotic area has disappeared into an empty network, with only residual cell fragments and infiltration of inflammatory cells mainly composed of neutrophils visible.

　　(3) Comparative medical ischemia-reperfusion injury is a common tissue and organ injury in clinical surgery, which plays an important role in the pathological evolution of diseases such as severe infections, trauma, shock, and cardiopulmonary dysfunction. The pathogenesis of ischemia-reperfusion injury in the body is mainly believed to be related to the generation of large amounts of oxygen free radicals during ischemia, which leads to lipid peroxidation in tissues and organs associated with ischemia. Due to the distribution characteristics of liver and intestinal blood vessels in the body, intestinal ischemia-reperfusion can cause liver injury and even lead to multiple system organ dysfunction syndrome. The mouse ischemia-reperfusion liver injury model replicated by this method should be kept warm and moist during the model making process. After closing the abdominal cavity, the abdomen should be covered with gauze, and physiological saline should be added to the gauze in a timely manner. This model can simulate ischemia-reperfusion liver injury in clinical patients and has practical value in exploring the pathogenesis and drug intervention of ischemia-reperfusion injury.