动物造模,药效评价,DIC模型 z-bio 2024-08-21 446

　　(1) The replication method involves injecting 250 μ g of pathogenic Escherichia coli endotoxin into the ear vein of male New Zealand rabbits weighing around 2kg. After 24 hours, inject the same dose again through the ear vein. Observe and record the performance after each injection. After the second injection of endotoxin for 4 hours, the rabbits were euthanized by injecting air into the ear vein and immediately dissected for visual observation. Take kidney tissue for rapid frozen section, perform HE staining, take kidney, lung, and liver tissue for paraffin embedded section, perform HE staining, phosphotungstic acid hematoxylin staining, and Gram staining respectively, and observe under an optical microscope.

　　(2) The characteristics of this model are that it can induce DIC lesions and significant pathological changes similar to those in humans in rabbits. The method is simple, the results are reliable, and it is easy to observe. It can be used for general teaching and scientific research applications.

　　(3) After the first injection of endotoxin in this comparative medicine model, the mononuclear macrophage system function of the model animals was impaired, and the body's ability to clear infectious toxins, activated coagulation factors, fibrinogen degradation products, etc. was reduced, allowing the second injection of endotoxin to better induce DIC. This is one of the advantages of this experimental method. The later stage of DIC disease development is secondary hyperfunction of the fibrinolytic system, and most of the dissolution disappears in micro blood tests. We euthanized the animals 4 hours after the second injection of endotoxin, and the detection rate of micro thrombus was high, without obvious hemolysis or bleeding. This is the second advantage of our experimental method over other methods. The mechanism of DIC formation caused by endotoxins is complex and the result of multiple factors. It induces DIC lesions similar to those in humans in rabbits and presents significant pathological changes.