动物造模,药效评价,原发性癫癎 z-bio 2024-08-21 370

　　(1) The replication method determines the degree of seizures in mice before the experiment, and selects qualified animals for the experiment. Place the animal on a small wooden board and gently throw it 10-15 cm into the air. Repeat this process several times to several times, and it will trigger an attack. This difference is due to the different seizure thresholds of each mouse. The general rule is to repeatedly toss it up to 80 times without sending it to the author, which is considered an insensitive mouse and not used for experiments.

　　(2) The seizures of epileptic like mice can be divided into two phases: the burst phase and the late burst phase. During the process of throwing into the air, if the mouse suddenly makes a chirping sound, it should immediately stop throwing and observe carefully. The mouse quickly enters a temporary immobility state, with its tail curved upwards towards its back, and then runs and enters an explosive epileptic state; Subsequently, in the later stage of the outbreak, the mice maintained a kangaroo posture, with their tails raised, their forelimbs lifted, and they engaged in spasms, chewing activities, and head turning to the side. They also experienced balance disorders, salivation, and urinary and fecal incontinence. The entire seizure process lasts for 13-20 seconds, and then enters a convulsive refractory period of about 30 minutes, during which any stimulation does not cause a seizure.

　　(3) Comparative medicine epilepsy like mice are purebred mice obtained through 12 generations of inbreeding of DDY mice, and are internationally recognized as a genetic epilepsy model. This type of mouse triggers seizures when stimulated. For example, repeatedly throwing, shaking, and rotating it into the air can cause seizures. Animals begin to have seizures at 5-8 weeks after birth, reaching their peak of sexual maturity and lasting for a lifetime thereafter. The initial site of epileptic seizures in mice is in the hippocampus and deep temporal lobe, and then spreads to other parts of the brain. Its seizure manifestations are similar to those of human temporal lobe epilepsy in many ways, making it a model of temporal lobe epilepsy.