动物造模,药效评价,颅内高压 z-bio 2024-08-21 436

　　(1) Method of replication: Healthy domestic dogs weighing 10-20kg. Administer pentobarbital sodium intravenously at a dose of 30mg/kg body weight for anesthesia. Then endotracheal intubation is performed to maintain unobstructed breathing, and femoral artery intubation is performed to monitor and record blood pressure and heart rate. Perform lumbar puncture on the lumbar sacral segment and measure the cerebrospinal fluid pressure in the lumbar region through a catheter. Remove hair from the top of the head and disinfect the skin in the surgical area. Drill bilateral holes 1-1.5cm along the midline of the skull and 0.5cm before the coronal suture. Slowly insert a latex balloon with an inner diameter of 2.0mm and a natural volume of 2ml into one side of the epidural space, and connect the syringe with a silicone tube to increase intracranial pressure. Drill a hole on the other side and insert a ventricular tube. When the plastic tube is gradually filled with cerebrospinal fluid, fix and connect a pressure sensor to measure intracranial pressure. Both sides of the skull drilling defects are sealed with bone cement or self setting dental powder. Slowly inject 37 ℃ physiological saline into the latex balloon through a silicone tube to maintain intracranial pressure above (8 ± 0.4) kPa. During the experiment, maintain the animal's body temperature at 37-38 ℃.

　　(2) The model features the same characteristics as the rabbit epidural balloon injection method, simulating intracranial hypertension caused by acute intracranial space occupying lesions. Experimental designers can analyze corresponding pathological indicators as needed. Blood can also be collected at different time points when intracranial pressure increases for blood gas analysis, and brain slices can be subjected to histopathological analysis.