动物造模,药效评价 z-bio 2024-08-09 390

　　1. Modeling material animal: rabbit, weighing about 2.5kg, both male and female; Medications: Chloralose, Uratan, Chlorosuccinylcholine; Equipment: Biological function experimental system, stereotaxic instrument.

　　2. Modeling method: Animals were anesthetized by intravenous injection of 0.05g/kg chloralose and 0.5g/kg Uratan. Tracheal intubation and artificial respiration were performed, followed by intravenous injection of 5-10mg/kg of succinylcholine to relax skeletal muscles. Half of the dose was supplemented every 0.5-1 hour to maintain skeletal muscle relaxation, and the left common carotid artery was isolated for future use. On the animal fixation and stereotaxic device, insert the stainless steel bipolar stimulation electrode into the inner amygdala nucleus (AME) on the right side according to the Sawger diagram, with coordinates A: 10, R：2.8，H：6.5， The electrical stimulation is generated by the 57-6 type electric pulse stimulator. The stimulation is a continuous wave with a frequency of 60Hz, an intensity of 20-30V, a stimulation time of 10s, and a stimulation interval of 10-20 minutes. The stimulation is repeated 2-4 times, and stable arrhythmia can occur.

　　3. Modeling principle: Electrical stimulation of the central nervous system induces arrhythmia and establishes an animal model.

　　4. After modeling, repeated electrical stimulation 2-4 times can result in stable arrhythmia. The myocardial contractility generated by various ectopic rhythms is lower than that of sinus rhythm, and rhythm affects myocardial contractility. The physiological maximum shortening speed is linearly correlated with the R-R interval of electrocardiogram. The supraventricular premature contractions produced during sinus bradycardia may have a greater contractility than those of the preceding sinus rhythm.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections, surgical trauma should be minimized, and aseptic operation should be strictly enforced.