动物造模,药效评价,肺炎 z-bio 2024-08-06 298

　　(1) Method of replication: Select a small amount of mucinous PA colonies stored at 4 ℃, incubate them in MH broth at 37 ℃ for 24 hours, and prepare a bacterial suspension of 0.5 units using MH broth. Freshly prepare on the same day of use. New Zealand rabbits aged from 18 to 22 months and weighing about 2kg were taken, and 0.5 McIntosh units of fresh PA viable bacterial suspension 1ml (equivalent to 1.5 × 100000000 CFU/ml) was inhaled through a spray every time. The rabbits were infected 3 times a week for 4 weeks. After inhaling Pseudomonas aeruginosa, animals may experience significant chills, fever, and difficulty breathing in the early stages, followed by dull hair, delayed response, poor nutritional status, and a significant increase in the total number of white blood cells in the blood count.

　　(2) Characteristics of the model The chronic pulmonary infection model of PA prepared by using mucus PA to continuously infect old rabbits through a spray is simpler and more convenient than that of artificial biofilm coated PA, but the amount of bacteria inhaled is not easy to control, and the modeling time is long.

　　(3) In clinical comparative medicine, pulmonary infections caused by PA in humans are more common in the elderly, often secondary to chronic lung diseases. During the modeling process of this method, when elderly rabbits inhaled PA bacterial solution, most animals showed obvious symptoms of bacterial infection: high fever, increased total peripheral blood white blood cell count, and lung lesions

　　Severe, with early acute inflammatory reactions such as bleeding, exudation, neutrophil infiltration, and formation of small lung abscesses, manifested as pathological features of acute bacterial infection. As bacteria are repeatedly inhaled, more chronic inflammation and acute pathological changes occur on the basis of chronic inflammation. After stopping inhaling bacterial liquid, this chronic inflammatory change persists and does not disappear for a long time.