动物造模,药效评价,HGV感染 z-bio 2024-08-05 401

　　(1) The model was made using experimental rhesus monkeys weighing 2.5-6.5kg and aged 2-8 years. Prior to infection, ALT enzyme activity was detected to be normal, and there were no dynamic liver pathological changes observed in liver biopsy. Intravenous injection of human HGV carrier serum into rhesus monkeys at a dose of 0.5ml/animal; On the 8th day, repeat the vaccination with 0.5ml of the same positive serum. Within 4 months after vaccination, venous blood will be collected once a week, and then every other week, 5ml of blood will be collected each time for HGV-RNA, HGV IgG, and serum transaminase (ALT) testing; At the 10th week, liver biopsy was performed under anesthesia with Su Mian Xin injection. After fixation, routine tissue sections were made and dynamically observed under light microscopy.

　　(2) Model characteristics: In the first week after vaccination and infection, experimental monkeys began to show positive HGV-RNA, and in the second week, HGV IgG antibody was positive. Both indicators remained positive for more than 50 weeks, and serum ALT detection was within the normal range. At the 10th week after inoculation with HGV, microscopic histopathological observation showed swelling of liver cells in the liver tissue, infiltration of lymphocytes in the local liver lobule parenchyma, and very few punctate necrosis were observed in the liver lobules. A small amount of mononuclear cell infiltration appeared around the punctate necrosis, and lymphocytes and granulocytes infiltrated some areas of the liver tissue, indicating mild acute hepatitis lesions; At week 20, the cytoplasm of liver cells in the liver tissue became loose, appearing as balloon like changes, with increased punctate and focal hepatocyte necrosis. Some areas showed infiltration of lymphocytes and monocytes, and some areas showed significant infiltration of eosinophils, accompanied by a chronic trend.

　　(3) After being vaccinated with HGV in comparative medicine, the infection rate of rhesus monkeys can reach 100%; And the key indicators such as serology, immunology, and liver histology of infected monkeys have been positive for a long time, but the ALT changes in the model monkeys after vaccination are not significant. The changing characteristics of HGV infection in model monkeys are similar to those of clinical human hepatitis G. The animal model established using rhesus monkeys with pathological characteristics similar to those of hepatitis G patients is of great significance for the study of human hepatitis G.