动物造模,药效评价,转基因动物肝癌 z-bio 2024-08-02 353

　　The modeling mechanism involves introducing specific exogenous genes into the genome of animals through genetic engineering methods, allowing for integration and genetic reproduction, and thus cultivating animals carrying exogenous genes. The imported exogenous gene, hepatitis B virus X (HBx), encodes a multifunctional protein with transactivation function. It plays an important role in regulating gene transcription, cell signal transduction, cell proliferation, and transformation, and is closely related to the occurrence of liver cancer; SV40 can encode two oncogenic proteins (T-Ag and t-Ag), which cause cancer by inactivating host cell tumor suppressor genes (such as p53 and Rb). TGF - β 1 can accelerate the development of liver injury and fibrosis.

　　[Modeling Method] Experimental animals are mostly CD1, C57BL/6 × DBA, C57BL/6 × SJL, etc. Exogenous genes transferred into animal bodies can be single genes (such as HBx, myc, SV40, etc.) or double genes (such as c-myc/TGF alpha, c-myc/TGF - β 1, HBx/myc, etc.).

　　The success rate of animal models with single gene transfer is higher than that with double gene transfer, but the time required for double gene transfer is significantly shortened, which is beneficial for research. The process of establishing this model itself can be used to study the pathogenesis of liver cancer. In addition, this model can not only study the overall tissue and organ level, but also the cellular and molecular level, making it an ideal model for the pathogenesis and drug screening of liver cancer.

　　[Model Evaluation and Application] Transgenic animal models are suitable for studying the role of genes in the formation of liver cancer and the multi-step process of hepatocellular carcinoma development. They provide an ideal experimental system for the pathogenesis, drug screening, and clinical medical research of liver cancer. However, their disadvantages include that gene mutations already exist during embryonic formation, which may activate compensatory molecular pathways, and the production technology requires high technical requirements and relatively high prices.