动物造模,药效评价,脑卒中 z-bio 2024-08-01 436

　　Cerebral infarction belongs to the category of "stroke" in traditional Chinese medicine and is a serious disease that threatens human health. Qi deficiency and blood stasis are one of its important pathological mechanisms. The methods for replicating animal models of stroke both domestically and internationally are basically in the acute phase of stroke. For post-stroke sequelae, neither animal models have been replicated, nor have they been combined with traditional Chinese medicine dialectics.

　　[Modeling mechanism] A rat model of right middle artery infarction was created by craniotomy, and a composite model of qi deficiency and blood stasis syndrome was established by continuous stimulation of rats through reperfusion and random electric pulse stimulation.

　　After anesthesia in rats, the left common carotid artery was isolated and ligated, and then the right common carotid artery was isolated and ligated for 1 hour before being released. Make a 1.5cm incision on the line connecting the right ear margin and eyelid, separate the temporal muscle, expose the skull, drill a bone window through the exposed bone, block the middle cerebral artery with electrocoagulation, and use a rat random pulse stimulator to stimulate for 30 days, with a stimulation intensity of 50-80V, 0.5-25Hz, 0.1-1.1 milliseconds. Observation indicators: rat performance, neurological deficit score, brain tissue pathological section, energy metabolism, etc.

　　【 Model Characteristics 】 After stimulation, rats showed dull and curly hair, decreased mental state, fearful expression, emaciation, limb weakness, reduced food intake, or left upper limb adduction weakness. When climbing, leaning to one side, difficulty in abduction and adduction of lower limbs, limping when walking or jumping, unable to curl the body normally when lifting the tail of the mouse, can only keep shaking the body, and the left testicle of male mice atrophies with uneven sizes on both sides.

　　【 Model Evaluation and Application 】 A pathological model of "Qi Deficiency and Blood Stasis" was developed in rats with post-stroke sequelae using a multifactorial composite method. Significant obstacles were observed in energy metabolism, and the traditional Chinese medicine syndrome of "Qi Deficiency" mainly manifested as weakness in propulsion, biochemical deficiency, and metabolic obstruction, thereby affecting organ function. This provides an objective basis for judging the indicators of "Qi Deficiency" in the Qi Deficiency and Blood Stasis syndrome model of cerebral infarction (stroke). This model is used for traditional Chinese medicine research on the pathology and physiology of cerebral infarction.