动物造模,药效评价,脾切除后肺炎球菌感染 z-bio 2024-07-25 374

　　1. Modeling material animals: Wistar rats, male or female, weighing 115g-125g; Drug: Type III pneumococcal; Instrument: Ultrasonic atomizer.

　　2. Modeling method: After splenectomy, animals were fed under the same conditions for 6 months for experimental observation.

　　Cultivate pneumococcal bacteria according to conventional methods, centrifuge and discard the supernatant, wash twice with PBS (0.01mol/L, pH 7.2), measure the A value with a spectrophotometer, adjust the bacterial suspension concentration to 5 × 10 ^ 9 cells/ml, and dilute it by multiple to verify the colony forming unit (CFU) value of the blood plate culture count.

　　Add the bacterial suspension to an ultrasonic atomizer and nebulize it at a rate of 1ml/min into the nebulization chamber. The animal is placed in the nebulization chamber and self inhales pneumococcal bacteria for 30 minutes.

　　3. The principle of modeling is to increase the susceptibility of splenectomy animals and establish a model of pneumococcal infection in animals by nebulization inhalation.

　　4. Changes after modeling: After inhaling pneumococcal bacteria, the modeling rats showed symptoms such as poor mental state, reduced activity, rapid breathing, erect body hair, and "cuckoo" sounds during breathing; Histological observation shows that the alveoli of the model animals are filled with a large number of red blood cells and relatively few neutrophils; Autopsy also revealed bleeding from one or both adrenal glands. The viable bacterial count in the peripheral blood of the model animals reached (1.62 × 100000 ± 142)/ml.