动物造模,药效评价,急性髓性白血病 z-bio 2024-07-16 312

　　[Modeling mechanism] The human acute myeloid leukemia cell line HL-60 was allogeneic and transplanted into SCID mice with T and B lymphocyte function combined with immune deficiency to establish a SCID mouse model with human hematopoietic function. This is currently the most commonly used animal model for studying human myeloid leukemia.

　　【 Modeling Method 】 Prepare a suspension of HL-60 cells and inject them into the tail vein of SCID mice weighing 15-20g for 4-5 weeks. The injection volume of cells is 6 × 10000000-7 × 10000000 per mouse, and the wound can be stopped by pressing and stopping the bleeding.

　　【 Model Characteristics 】 ① Mice develop disease quickly, with infiltration of leukemia cells in peripheral blood visible within 3 weeks, and the proportion of leukemia cells in peripheral blood can gradually reach 3% to 11%. Randomly selected mice undergo bone marrow examination, and the proportion of leukemia cells in bone marrow reaches the diagnostic rate of human leukemia (i.e.>30%). The mice showed symptoms similar to human leukemia, such as poor mental state, reduced activity, unstable gait, slow movement, and decreased appetite. Some mice formed solid tumors in the abdominal cavity SCID mice can rapidly spread and develop disease by intravenous or intraperitoneal inoculation of HL-60 cells. After intraperitoneal inoculation, tumor cells can directly infiltrate surrounding tissues and organs; Intravenous vaccination manifests as varying degrees of infiltration of leukemia cells into various organs throughout the body, with a large number of leukemia cells appearing in peripheral blood, which is very similar to the clinical manifestations of human leukemia.

　　[Model Evaluation and Application] This model is currently a widely used animal model for studying human acute myeloid leukemia. Mainly used for research on the mechanisms and pharmacological effects of disease occurrence and development.