动物造模,药效评价,血管性痴呆 z-bio 2024-07-04 304

　　1. Animal modeling materials: SD rats, male, weighing 350-400g; Medications: Aseptic naturally dried blood clots of the same type in rats, physiological saline, chloral hydrate, and scopolamine.

　　2. Modeling method: Take sterile and naturally dried blood clots of the same type of rat, grind them and pass them through a 200 μ m sieve for later use. When using, 0.5ml of physiological saline was taken and 100mg of blood clot was homogenized to form a suspension. The diameter was measured under a microscope, with a diameter of 40-200 μ m, mostly 150-200 μ m.

　　Rats were anesthetized with chloral hydrate intraperitoneally. The common carotid artery was exposed through a midline incision of the neck, temporarily clamped, and retrograde catheterization was performed at the bilateral external carotid arteries. After injecting 0.5ml of embolic saline, the external carotid artery was immediately ligated, while the common carotid artery was opened to allow the embolus to enter the skull and brain through the internal carotid artery, forming a multifocal cerebral infarction. After surgery, 2.5mg/kg of scopolamine was subcutaneously injected daily for one week to promote the formation of intellectual disabilities.

　　3. Modeling principle: Vascular dementia, also known as cerebral infarction dementia, is established by causing cerebral vascular embolism in animals to establish an animal model of vascular dementia.

　　4. After modeling, the results of the dark avoidance reaction experiment showed that the learning and memory abilities of the vascular dementia rat model group were significantly lower than those of the normal control group, indicating that the intelligence of the animal model caused by this method was significantly reduced, and the animals showed certain signs of dementia.

　　5. Biochemical changes after modeling

　　(1) Rat lactoperoxidase (LPO) levels: The LPO levels in the cortex, thalamus, hippocampus, and striatum of the normal group were (74.5 ± 43.1), (98.1 ± 36.9), (34.8 ± 12.7), and (35.2 ± 12.0) nmoL/100mg, respectively. The LPO levels in the cortex, thalamus, hippocampus, and striatum of the model group were (171.7 ± 45.1), (257.2 ± 46.3), (102.6 ± 23.0), and (96.3 ± 24.1) nmoL/100mg, respectively.

　　(2) SOD levels in rats: The SOD levels in the cortex, thalamus, hippocampus, and striatum of the normal group rats were (32.0 ± 8.9), (13.8 ± 2.7), (3.9 ± 1.5), and (23.8 ± 4.0) ng/ml, respectively. The SOD levels in the cortex, thalamus, hippocampus, and striatum of the model group rats were (10.2 ± 7.5), (11.4 ± 3.7), (1.8 ± 1.6), and (13.1 ± 5.7) ng/ml, respectively.

　　Compared with the control group, the vascular dementia model group showed an increase in LPO and a decrease in SOD activity.

　　6. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections. Surgical trauma should be minimized and sterile operations should be strictly carried out.