动物造模,药效评价,心肌缺血 z-bio 2024-07-02 516

　　Method 1

　　1. Animal modeling materials: rats; Medication: Pituitrin; Equipment: Biological function experimental system.

　　2. Method of modeling: Rats were intravenously injected with 0.5u/kg or 11u/kg of pituitrin, with a volume of 1 or 1.5ml, and the injection was completed within 10 or 15 seconds.

　　3. Modeling principle: Pituitrin causes myocardial ischemia in animals.

　　4. Changes after molding: The T-wave rises from 5 to 20 seconds after molding, the S-T segment rises, and even shows a unidirectional curve. The T-wave decreases, becomes flat, biphasic, or inverted 30 seconds to a few minutes after injection, and there is no significant change in the S-T segment.

　　5. Precautions: Animals should choose those with a rising J point or ST segment that is raised or lowered by more than 0.1mV, and those with T waves that are initially raised and then flattened or inverted for experimental use. The J point is the junction of the ST segment at the end of the QRS complex, known as the junction point (J point). The weight difference of experimental animals in the same batch should not be significant.

　　Method 2

　　1. Animal modeling materials: Wistar rats, 18-20 months old, male; Medication: 20% Ulatan, Pituitrin; Equipment: Biological function experimental system.

　　2. Method of modeling: Rats were anesthetized by intraperitoneal injection of 20% Ulartan (5ml/kg) and fixed on a fixed plate in a supine position. They were allowed to stand for 15-20 minutes (based on the basic stability of the electrocardiogram), and a dose of 20u/kg of posterior pituitary hormone was rapidly injected from the abdominal cavity. Immediately observe the changes in ST Ⅱ and T Ⅱ on the electrocardiogram, with T wave elevation, S-T segment elevation (≥ 0.1mV), and significant slowing of heart rate as the criteria for successful modeling.

　　3. Modeling principle: Pituitrin causes myocardial ischemia in animals.

　　4. After modeling, observe the changes in electrocardiogram ST Ⅱ and T Ⅱ. The model group animals had high T waves, elevated S-T segment (≥ 0.1mV), and significantly slowed heart rate.

　　5. After modeling, the biochemical changes showed that the active reactants of peroxidase were brown, and the cytoplasm, plasma membrane, and blood cells of normal myocardial tissue were darker in color, showing strong positive reactions; The myocardial fibers are uniformly colored in a light brown color, with weak positive reactions. The experiment observed that in the normal group, the cytoplasm, plasma membrane, and blood cells of the myocardial tissue were deeply stained, and the positive reactants were arranged in a linear pattern along the long axis of the myocardial cells; The myocardial fibers are uniformly light brown in color, with no obvious horizontal stripes. The cytoplasm, plasma membrane, and blood cell staining of the myocardial tissue in the model group were lighter than those in the normal group, and the positive reactants were sparsely distributed in a dotted pattern; The myocardial fibers are uniformly light brown in color, with no obvious horizontal stripes.

　　60 minutes after intraperitoneal injection of posterior pituitary hormone into the model group, the peroxidase activity was significantly reduced compared to the normal group (46.38 ± 3.86 in the normal group and 12.38 ± 1.29 in the model group).