动物造模,药效评价,慢性肝损伤 z-bio 2024-07-01 330

　　1. Animal modeling materials: Rats, weighing 200-250g, regardless of gender; Medication: Liquid feed containing total nutrients and alcohol (Lieber Decarli liquid feed).

　　2. Modeling method: Feed rats with liquid feed containing total nutrients and alcohol. This food is formulated based on calories, with 21% fat, 18% protein, 47% carbohydrates (36% of which are replaced by alcohol), as well as some vitamins and inorganic salts. Solid food and water are not given, and it is fed for 4 weeks or more.

　　3. Principle of modeling: Alcohol causes chronic liver damage.

　　4. Changes after modeling: Animals in the modeling group showed significant hepatic steatosis after being fed for about 4 weeks, but no alcoholic hepatitis or fibrous tissue proliferation was observed. Serum ALT and AST levels were elevated.