动物造模,兔肺炎模型,药效评价 z-bio 2024-07-01 279

　　1. Animal modeling materials: New Zealand white rabbits, weighing 1.7-2.3kg; Drug: Escherichia coli.

　　2. Method of modeling: Escherichia coli is seeded on a nutrient agar slope and stored in a refrigerator at 4 ℃. Before use, it is picked up with an inoculation ring on a sterile operating table and transferred to a regular broth medium. It is incubated at 37 ℃ for 48 hours and diluted with physiological saline for counting. The concentration is dropped to 3.8 × 1000000 cells/ml.

　　The animal in the modeling group is fixed on the rabbit platform, and the front teeth are pulled with a thin rope to fully expose the neck. Cut off the rabbit hair around the neck, disinfect with 75% alcohol, insert the syringe for extracting Escherichia coli solution through the skin into the trachea under the cricoid cartilage, and inject 0.8-1.2ml/kg of bacterial solution after a large amount of bubbles are found during the suction. Then, lift the rabbit's head and neck to allow the bacterial solution to slowly flow into the lower part of the trachea and lungs.

　　3. Principle of modeling: Escherichia coli infection leads to animal pneumonia.

　　4. Changes after modeling: The animals in the modeling group sneezed, throated, refused to eat, raised their hair, curled up, and trembled for 1-3 hours after inoculation. After 5-6 hours, the body temperature rises, and the furrowing, curling, and trembling disappear, and drinking water begins. After 12 hours, the body temperature significantly increased, breathing increased rapidly, and restlessness occurred. Some animals exhibit cyanosis. Pathological examination shows obvious congestion, edema, stasis, bleeding or bleeding in the lungs, with spots and patches spreading across both lobes of the lungs. The alveolar cavity in the lesion area is filled with a large number of neutrophils and a few eosinophils. Inflammated bronchioles can be seen in the center or periphery of the lesion, with congested walls and a small amount of infiltration of neutrophils and eosinophils. Partial mucosal epithelial cells necrosis and shedding.