动物造模,药效评价 z-bio 2024-06-19 596

　　【 Modeling Mechanism 】 Human SCC tissue or cancer cells can be inoculated into the back, forelimb armpits, groin area, lateral chest wall, or sublingual area of immunodeficient mice to produce SCC locally.

　　【 Model characteristics 】 Currently, commonly used animals include nude mice, C3H, ICR, NC, BALB/c, C57BL/6, SCID, NOD/SCID mice, etc. In addition, rats, guinea pigs, etc. can also be used for model construction. Someone implanted tongue squamous cell carcinoma tissue into the tongue of nude mice and found that the lymph node metastasis rate was 21% at the 4th week; After 20 weeks, the lymph node metastasis rate increased to 79%. Tca8ll3 cells were injected into different parts of the tongue, floor of the mouth, and armpit of nude mice, and it was found that the cells injected into the bottom of the mouth of the nude mice had strong cervical lymph node metastasis.

　　The non homologous tongue SCC model established using New Zealand rabbits showed a tumor formation rate of 100%, lymph node metastasis rate and lung metastasis rate of 50% and 20%, respectively. The tongue squamous cell carcinoma cells of New Zealand rabbits exhibit highly malignant and poorly differentiated characteristics, and their biological characteristics are very similar to those of human tongue squamous cell carcinoma cells.

　　A tongue SCC model of rabbits was established through chemical induction, and the primary tumor tissue of the model was transplanted subcutaneously into nude mice or under rabbit skin to generate tumors again. It is also possible to construct a visualized animal model of skin squamous cell carcinoma as a whole, which can clearly observe the occurrence, development, and infiltration of subcutaneous tumors in the non-invasive state of living experimental animals.

　　Model evaluation and application: The tongue SCC model is simple to construct and easy to repeatedly observe the biological characteristics of SCC, which has advantages. However, due to the complex anatomical relationships of the neck lymphatic system in rodents, the lymphatic reflux areas are crisscrossed, which affects the sampling of lymph nodes; Moreover, oral injection modeling affects animal feeding, so although rodent tongue squamous cell carcinoma models are suitable for studying metastasis pathways and mechanisms, they are not suitable as animal models for quantitative detection of metastatic cancer cells and drug intervention experiments.

　　The overall visualization animal model of skin SCC is simple and easy to implement, with a high success rate. In drug therapy, the dose-response relationship between drugs and tumor changes can be dynamically and real-time observed, and changes in various indicators such as serology and immunology can be obtained in real-time, which has important application value for studying skin cancer treatment.