动物造模,慢性支气管炎,药效评价 zi-bio 2024-05-16 921

　　1. Animal modeling materials: Male SD rats, weighing 150-180g; Medications: sawdust, tobacco, sulfur, chili; Equipment: Smoke room.

　　2. Modeling method: Animals were divided into four groups and exposed to stimuli for 1, 3, 5, and 7 weeks, respectively.

　　In a 15m3 smoke chamber, 110g of sawdust, 10-11g of tobacco leaves, 3-4 g of chili peppers, and 0.4-0.6g of sulfur were burned for 30 minutes at a concentration of 200ng/m3, once a day, six times a week, for a total of 7 weeks, to establish a chronic branch organ series development model. Animals with interstitial pneumonia were excluded.

　　3. The principle of modeling is to establish a chronic bronchitis model by inhaling smoke to cause tracheal injury and inflammation in animals.

　　4. histopathological changes after modeling: The formation of chronic bronchitis caused by smoke shows a gradual development process. By the end of week 7, the animal's airway exhibits typical histopathological changes, including obvious lodging of bronchial mucosal epithelial cilia, epithelial shedding or ulceration, increased secretion in the lumen, infiltration of inflammatory cells, goblet cell proliferation, and thickening of the tube wall.

　　During the formation of chronic bronchitis, the total number of inflammatory cells and the percentage of lymphocytes show an increasing trend, while the percentage of macrophages gradually decreases. The percentage of neutrophils remains at a high level at one week and then remains at a low level, with no significant difference compared to the control group.