动物造模,慢性支气管炎,药效评价 zi-bio 2024-05-16 828

　　1. Animal modeling materials: mice; Medication: Cigarette; Instrument: Lower mouthed bottle.

　　2. Modeling method: The mice in the modeling group were placed in a 10000ml lower mouthed bottle with a 1.5cm diameter vent on the bottle cap. A three-way tube was connected to the lower mouthed bottle, and a 50ml syringe and lit cigarettes were connected to the other two ends. The syringe was used to continuously inject cigarette smoke through the three-way tube, with 400ml (the concentration of smoke in the bottle is about 4%) smoked for 30 minutes each time. Smoke once in the first 10 days and afternoon respectively, and smoke once in the afternoon every day for the next 10 days, for 30 consecutive times for a total of 20 days.

　　3. The principle of modeling is to establish a chronic bronchitis model by inhaling smoke to cause tracheal injury and inflammation in animals.

　　4. Changes after modeling: Chronic bronchitis lesions appear in modeling animals, with goblet cell proliferation, columnar epithelial proliferation, and chronic inflammatory cell infiltration being the most common.