动物造模,慢性心衰 zi-bio 2024-04-28 1277

　　Objective: To compare animal models prepared with three different regimens and explore a stable, reliable, and reproducible mouse model of chronic heart failure.

　　Method: Twenty five male C57BL/6J mice were randomly divided into four groups: normal group (ZC group), experimental group A (MA group), group B (MB group), and group C (MC group). The experimental group adopted different preparation schemes for continuous injection of ISO, with the MA group and MB group using a concentration decreasing modeling method. The MA group (10mg/kg on day 1, 5mg/kg on day 2, 2.5mg/(kg · d) on days 3-30); Subcutaneous injection for 30 days; MB group (20mg/kg on day 1, 10mg/kg on day 2, 5mg/(kg · d) on days 3-14); Subcutaneous injection for 14 days; Construct an animal model of chronic heart failure in the MC group (with a constant concentration of 7.5mg/(kg · d) and intraperitoneal injection for 28 days). On the second day after injection, calculate the survival rate and modeling rate of each group of mice. Detecting cardiac function through echocardiography and measuring NT ⁃ proBNP, IL-6, TNF ⁃ in serum using ELISA α  Horizontal.

　　After the injection on the 30th day, although all experimental groups were able to effectively induce chronic heart failure, it was found that the modeling situation of the 7.5mg/kg concentration MC group was the most stable, which is more suitable for subsequent research on heart failure related to traditional Chinese medicine.

　　Conclusion: The optimal protocol for preparing a mouse chronic heart failure model using ISO is a constant 7.5mg/(kg · d) and continuous intraperitoneal injection for 28 days.