动物造模,脓毒症 zi-bio 2024-04-28 539

　　Objective: To explore the changes in coagulation function and inflammation levels during the course of sepsis.

　　Method: 48 male SD rats were randomly divided into a blank group (Control group, n=8), a sham surgery group (Sham group, n=8), a compound infection sepsis model 4h (4h group, n=8), 8h (8h group, n=8), 12h (12h group, n=8), and 16h (16h group, n=8) groups, using an improved cecal ligation and puncture technique to construct a compound infection sepsis rat model. Inflammatory and coagulation related indicators were detected.

　　Result: (1) The levels of lipopolysaccharide and interleukin-6 (IL-6) in all sepsis model rats were significantly higher than those in the Sham group (P<0.001), and with prolonged postoperative time, the levels of LPS and IL-6 gradually increased. After 12 hours, there was no significant change in LPS; (2) The prothrombin time in the middle and late stages of the sepsis model group (8 hours and later) was significantly longer than that in the Sham group (P<0.01); (3) Compared with the Sham group, the activation time of partial prothrombin in the 8h, 12h, and 16h groups was significantly prolonged (P<0.05, P<0.01), and after 8 hours, APTT gradually extended to approach the Control group; (4) After 8 hours (excluding 8 hours), the fibrinogen content significantly increased compared to the Sham group (P<0.01); (5) There was a significant difference (P<0.01) in the degradation products of fibrinogen between the control group and the control group at different time periods of sepsis, while there was no significant difference compared to the Sham group; (6) Antithrombobin III (AT III) levels were significantly lower in all time periods of sepsis compared to the Sham group (P<0.01), and AT III levels showed a decreasing trend with the course of the disease. There was a significant difference between the 4-hour and 8-hour groups and the 16h group.

　　Conclusion: The MIM rat model can better reflect the development trend and relationship between inflammation and coagulation disorders in the course of sepsis, and provide a research basis for exploring the development of sepsis.