动物造模,早发性卵巢功能不全 zi-bio 2024-04-28 960

　　Objective: To explore the changes in relevant indicators of early onset ovarian insufficiency (POI) mouse model induced by Tripterygium wilfordii glycosides, and determine the optimal time point for intervention measures.

　　Method: Forty female ICR mice were randomly divided into a control group, A, B, C, and D model groups, with 8 mice in each group. The control group was given pure water by gavage for 14 days (0.01mL/10g), while the other groups were given Tripterygium wilfordii glycoside suspension (80mg/kg, 0.01mL/10g) by gavage continuously for 1 day (A model group), 3 days (B model group), 7 days (C model group), and 14 days (D model group), and samples were taken in batches. Weigh the body weight, wet weight uterus, and bilateral ovaries of each group of mice; Enzyme linked immunosorbent assay was used to detect the serum levels of FSH, LH, E2, P, AMH, INH ⁃ B, and T in each group of mice; HE staining was used to observe the number and developmental status of follicles and corpus luteum at all levels in each group of mice; TUNEL fluorescence staining was used to detect the apoptotic area in the ovaries of mice in each group; IHC method was used to detect the positive expression of VEGFA, CD34, and EPO proteins in the ovaries of mice in each group; PCR method for detecting HIF-1 in each group of mice α、 MRNA expression levels of SDF-1 and CXCR4.

　　Compared with the control group, the changes in various indicators in Model A group did not meet the POI modeling criteria; The ovarian index, uterine index, and body weight of mice in the B model group were significantly reduced (P<0.01), the body weight of mice in the C model group was significantly reduced (P<0.01), and the ovarian index of mice in the D model group was significantly reduced (P<0.05); B. The serum indicators FSH and LH levels in the C and D model groups increased (P<0.05, P<0.01), while the levels of E2, PROG, AMH, INH ⁃ B, and T decreased (P<0.01); B. The number of primordial follicles, pre antral follicles, sinusoidal follicles, pre ovulatory follicles, and corpus luteum in the C and D model groups significantly decreased (P<0.05, P<0.01), while the number of atresia follicles significantly increased (P<0.01); A. The apoptosis area of TUNEL fluorescence staining significantly increased in model groups B, C, and D (P<0.05, P<0.01); B. The positive expression of CD34, VEGFA, and EPO decreased significantly in the C and D model groups (P<0.05, P<0.01); A. Model Group B HIF ⁃ 1 α、 The mRNA expression levels of SDF-1 and CXCR4 were significantly increased (P<0.05, P<0.01). Compared with the B model group, there were significant changes in the relevant indicators of the C and D model groups, indicating that the C and D models are more severe and tend to develop towards POF.

　　Conclusion: The B model group is a turning point in the progression of ovarian function from a damaged state of POI to irreversible premature ovarian failure (POF), indicating that three-dimensional administration of Tripterygium wilfordii glycosides is the best time to induce POI disease models and effectively intervene with drugs.